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RAS BiologyРадиационная биология. Радиоэкология Radiation biology. Radioecology

  • ISSN (Print) 0869-8031
  • ISSN (Online) 3034-5898

Bone-Marrow Compartment under Chronic γ- Irradiation Conditions ex vivo

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PII
S30345898S0869803125010019-1
DOI
10.7868/S3034589825010019
Publication type
Article
Status
Published
Authors
E. A. Markina
  • Affiliation: State Scientific Center of the Russian Federation - The Institute of Biomedical Problems of the Russian Academy of Sciences
P. I. Bobyleva
  • Affiliation: State Scientific Center of the Russian Federation - The Institute of Biomedical Problems of the Russian Academy of Sciences
O. V. Zhidkova
  • Affiliation: State Scientific Center of the Russian Federation - The Institute of Biomedical Problems of the Russian Academy of Sciences
P. V. Lashukov
  • Affiliation: State Scientific Center of the Russian Federation - The Institute of Biomedical Problems of the Russian Academy of Sciences
L. B. Buravkova
  • Affiliation: State Scientific Center of the Russian Federation - The Institute of Biomedical Problems of the Russian Academy of Sciences
Volume/ Edition
Volume 65 / Issue number 1
Pages
3-14
Abstract
Long-term exposure to ionizing radiation at low doses is one of the risk factors for astronauts’ health, at the same time, hematopoietic disorders caused by damage to bone marrow (BM) cells are the most common result of irradiation. The aim of this work was to evaluate the effects of chronic exposure to ionizing radiation on hematopoietic stem and progenitor cells (HSPCs) and BM stromal progenitor cells. In radiation exposure modeling, rats were exposed to 10-fold external fractionated gamma-irradiation at a total dose of 500 cGy for 33 days. The control group of animals was kept in standard vivarium conditions. The cellular composition and functional characteristics of rat femoral BM-derived cells were examined. A decrease in BM cellularity and changes in the expression of surface markers were observed after irradiation, which may indicate a disruption in the hematopoietic and non-hemopoietic cells communication. Stromal progenitor cells after irradiation were characterized by higher levels of induced and spontaneous adipogenic differentiation and reduced proliferative potential. The number of different hematopoietic colonies, except CFU-GM and the total number of colonies were decreased in the experimental group. After irradiation the culture of BM-derived cells was characterized by a higher production of cytokines, which inhibit HSPC proliferation (IL-18, IFNγ) and activate their differentiation (IL-6). There was also an increase in the expression of pro-resorptive genes and cytokines (Sost) along with a decrease in the expression of genes involved in osteogenesis. Thus, it was demonstrated that chronic fractionated irradiation in the low dose range causes negative changes in the stromal and hematopoietic BM compartment, which may lead to impaired hematopoiesis.
Keywords
ионизирующее излучение гемопоэтические стволовые и прогениторные клетки стромальные клетки-предшественники
Date of publication
20.02.2025
Year of publication
2025
Number of purchasers
0
Views
50

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